Challenge Room is a room in The Binding of Isaac.

The Challenge Room features 3 waves of enemies in exchange for a reward.

The room will only generate on a floor if Isaac's total health is greater than or equal to his number of heart containers. This is also the requirement to open the door.

Challenge Rooms appear only on the first floor of a chapter, or during Curse of the Labyrinth!, and may appear up to Chapter 5. Only one Challenge Room may generate per floor.

The room contains one or more random chests that, when interacted with, initiate the enemy waves.

The enemies inside the room depend on the current chapter.

Starting from Chapter 4, waves of bosses (typically from Chapters 1 or 2) will spawn instead of enemies.

Each completed wave will grant 1 charge to Isaac's active item and will trigger other room-clear effects such as familiars that spawn stuff based on the number of cleared rooms.

Completing all 3 waves will yield a regular room clear reward.

If Isaac teleports out of the room after it has been initiated, returning to the room will not continue the challenge unless another chest was left unopened, or an item pedestal has been spawned.

This page shows the latest information as of the new Repentance+ (Online Co-op) DLC.
To see information about this room for the previous Repentance version, check this page instead.

The Challenge Room is associated with the Treasure Room item pool. Items that spawn in this room will be selected from the associated item pool.

• Check out the room description on the external Isaac Wiki.