Challenges
| Name | Character | Starting items | Disabled rooms | Curse filters | Additional notes | End goal | Unlock condition | Reward |
|---|---|---|---|---|---|---|---|---|
| 1. [Epiph] Kagemane no Jutsu |  Tarnished Judas | Proptosis
9 Volt Spoon Bender | - | - | The Lamb | - | Thirty Pieces of Silver | |
| 2. [Epiph] NullPointerException | Tarnished Eden |  Glitched Photo | - | - | - | Mom's Heart | - | Drawn Card |
| 3. [Epiph] Pool! | Tarnished Cain | The Ludovico Technique (x2; bagged) | - | - | - | The Lamb | - |  Tasty penny |
| 4. [Epiph] Sweet Tooth |  Tarnished Magdalene | Sister Maggy
| - | - | Item pedestals are converted into large amounts of Red Hearts when touched, which disappear after some time or upon leaving the room. | Mom's Heart | - | Burning Passion |
| 5. [Epiph] One With Nothing | Tarnished Isaac | - | - | - | Tarnished Isaac starts with 12 slots.
Items will reroll into items with 1 quality lower instead of becoming Blight. | Isaac | - | Nothing |
| 6. [Epiph] Dealmaker | Tarnished Keeper | Stock Fluctuation | - | - | The price requirement of Turnover fluctuates similarly to Stock Fluctuation. | The Lamb | - | Coin Case |
| 7. [Epiph] Overwhelming Odds | Tarnished Isaac | Chance Cube
Lucky Cat | - | - | Tarnished Isaac starts with 12 slots.
All items are selected from random pools as if holding Chaos. Chance Cube is automatically used on occasion without consuming any charges. Soul of the Lost is triggered upon entering any Boss Room, and Crooked Penny in Treasure Rooms. | Mom's Heart | - | Chance Cube |
| 8. [Epiph] He Who Casts The First Stone |  Tarnished Samson | - | - | - | Tarnished Samson is unable to punch, but his slam attack charges twice as fast. | Isaac | - | Revolt |
| 9. [Epiph] Retribution |  Tarnished Lost | - | - |  Divine Remnants is always in its Level 1 form, and recharges 1 pip per 1.5 seconds. When fully charged, the Mantle effect breaks and triggers a Crack the Sky effect.
The effect will not reroll items or heart pickups. | Mom's Heart | - | Retribution | |
| 10. [Epiph] Emperor Of Flies | Tarnished ??? | Forever alone
The Swarm | - | Tarnished ??? starts with the Beelzebub transformation.
His poops are randomized at the start of every floor. Picking up Colostomy Bags will instead convert them into equivalent Fly Trap fly variants. | Mom's Heart | - | Fly Trap |